We present here the Book of Common Prayer in electronic form, formatted as the original. That is, it is intended to be as identical as possible to the U. S. BCP in appearance. It is hoped that parishes might find this useful, for example, in preparing special service booklets where unnecessary sections may be omitted, parts may be rearranged as needed so as to follow the actual order of service, or actual names (as in baptism or marriage) could be inserted. The text is in three word processing formats, RTF (Rich Text Format), which is recognizable by nearly all word processors, MSWord for Windows 97, and WordPerfect 6.0. The text is also available in Adobe Acrobat (PDF) files, which look more like the original, but are not generally editable. If you have Firefox, Internet Explorer, or a similar browser, clicking on one of the RTF or MSWord links below will either call up a "helper program" (WordPad if you have Windows) or give a message box. Just click on "Save to disk" (or something similar) in the message box or helper program, and the file will be saved onto your hard drive. For the PDF files, you will need the free Adobe Acrobat Reader. Once this is installed, clicking on a PDF file will automatically call up the Reader and you will be able to read the file, and save it onto your hard drive if you wish. If any errors are found please let us know. Suggestions for improvements are always welcome.
Tables for Finding the Date of Easter and other Holy Days 880 RTF (140K) MSWord (150K) PDF (210K) The Lectionary 888 Year A 889 Year B 900 Year C 911 Holy Days 921 Common of Saints 925 Various Occasions 927 Daily Office Lectionary 934 Seasons of the Year 936 Holy Days 996 Special Occasions 1000 Current Sunday and Daily Lectionary readings may be found at The Lectionary web site PDF (400K) RCL Sunday Lectionary (400K)
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Fonts: In order to minimize reformatting when using the RTF or WordPerfect files in a Word Processor, you will need to use the same font as was used in creating them. We have available for downloading a Garamond font for Windows which is both free (an important consideration!) and quite close in appearance to the font used in the U. S. Book of Common Prayer. It is a ZIP compressed file of four fonts of the Garamond family: normal, italic, bold, and bold italic. After downloading, you will need to uncompress it, and then install the files just as you would any other true-type font. Note: the MSWord files use the same Garamond font which is part of the standard MSWord/MSOffice installation.
Diverse approaches to mRNA cancer vaccines, including dendritic cell vaccines and various types of directly injectable mRNA, have been employed in numerous cancer clinical trials, with some promising results showing antigen-specific T cell responses and prolonged disease-free survival in some cases.
Although protein expression may be positively modulated by altering the codon composition or by introducing modified nucleosides (discussed below), it is also possible that these forms of sequence engineering could affect mRNA secondary structure32, the kinetics and accuracy of translation and simultaneous protein folding33,34, and the expression of cryptic T cell epitopes present in alternative reading frames30. All these factors could potentially influence the magnitude or specificity of the immune response.
Injection of naked mRNA in vivo. Naked mRNA has been used successfully for in vivo immunizations, particularly in formats that preferentially target antigen-presenting cells, as in intradermal61,65 and intranodal injections66,67,68. Notably, a recent report showed that repeated intranodal immunizations with naked, unmodified mRNA encoding tumour-associated neoantigens generated robust T cell responses and increased progression-free survival68 (discussed further in Box 2).
Intranasal vaccine administration is a needle-free, noninvasive manner of delivery that enables rapid antigen uptake by DCs. Intranasally delivered mRNA complexed with Stemfect (Stemgent) LNPs resulted in delayed tumour onset and increased survival in prophylactic and therapeutic mouse tumour models using the OVA-expressing E.G7-OVA T lymphoblastic cell line145.
All enzymes and reaction components required for the GMP production of mRNA can be obtained from commercial suppliers as synthesized chemicals or bacterially expressed, animal component-free reagents, thereby avoiding safety concerns surrounding the adventitious agents that plague cell-culture-based vaccine manufacture. All the components, such as plasmid DNA, phage polymerases, capping enzymes and NTPs, are readily available as GMP-grade traceable components; however, some of these are currently available at only limited scale or high cost. As mRNA therapeutics move towards commercialization and the scale of production increases, more economical options may become accessible for GMP source materials.
Once the mRNA is synthesized, it is processed though several purification steps to remove reaction components, including enzymes, free nucleotides, residual DNA and truncated RNA fragments. While LiCl precipitation is routinely used for laboratory-scale preparation, purification at the clinical scale utilizes derivatized microbeads in batch or column formats, which are easier to utilize at large scale156,157. For some mRNA platforms, removal of dsRNA and other contaminants is critical for the potency of the final product, as it is a potent inducer of interferon-dependent translation inhibition. This has been accomplished by reverse-phase FPLC at the laboratory scale158, and scalable aqueous purification approaches are being investigated. After mRNA is purified, it is exchanged into a final storage buffer and sterile-filtered for subsequent filling into vials for clinical use. RNA is susceptible to degradation by both enzymatic and chemical pathways157. Formulation buffers are tested to ensure that they are free of contaminating RNases and may contain buffer components, such as antioxidants and chelators, which minimize the effects of reactive oxygen species and divalent metal ions that lead to mRNA instability159.
.01 Paragraph .02 of AS 1001, Responsibilities and Functions of the Independent Auditor, states, "The auditor has a responsibility to plan and perform the audit to obtain reasonable assurance about whether the financial statements are free of material misstatement, whether caused by error or fraud. [footnote omitted]"1 This section establishes requirements and provides direction relevant to fulfilling that responsibility, as it relates to fraud, in an audit of financial statements.2
.12 As indicated in paragraph .01, the auditor has a responsibility to plan and perform the audit to obtain reasonable assurance about whether the financial statements are free of material misstatement, whether caused by fraud or error.7 However, absolute assurance is not attainable and thus even a properly planned and performed audit may not detect a material misstatement resulting from fraud. A material misstatement may not be detected because of the nature of audit evidence or because the characteristics of fraud as discussed above may cause the auditor to rely unknowingly on audit evidence that appears to be valid, but is, in fact, false and fraudulent. Furthermore, audit procedures that are effective for detecting an error may be ineffective for detecting fraud.
4 Intent is often difficult to determine, particularly in matters involving accounting estimates and the application of accounting principles. For example, unreasonable accounting estimates may be unintentional or may be the result of an intentional attempt to misstate the financial statements. Although an audit is not designed to determine intent, the auditor has a responsibility to plan and perform the audit to obtain reasonable assurance about whether the financial statements are free of material misstatement, whether the misstatement is intentional or not.
To stem the tide of incoming requests, help seekers, too, must change their behavior. Resetting norms regarding when and how to initiate e-mail requests or meeting invitations can free up a great deal of wasted time. As a step in this direction, managers at Dropbox eliminated all recurring meetings for a two-week period. That forced employees to reassess the necessity of those gatherings and, after the hiatus, helped them become more vigilant about their calendars and making sure each meeting had an owner and an agenda. Rebecca Hinds and Bob Sutton, of Stanford, found that although the company tripled the number of employees at its headquarters over the next two years, its meetings were shorter and more productive. 2ff7e9595c
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